Difference between revisions of "Part:BBa M50084"

 
 
Line 3: Line 3:
 
<partinfo>BBa_M50084 short</partinfo>
 
<partinfo>BBa_M50084 short</partinfo>
  
−
This part includes a Kozak, histidine tag, and serotonin receptor protein (5HT1A).
+
This part codes for a modified version of the 5HT1A receptor, a serotonin receptor taken from the human genome, codon optimized for expression in Saccharomyces cerevisiae. A HIS-tag was added at the beginning of the sequence on the extracellular side for detection purposes, chosen for its small size and the reduced likelihood that it would interfere in the receptor function.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:27, 5 December 2017


Yeast Serotonin Receptor Part

This part codes for a modified version of the 5HT1A receptor, a serotonin receptor taken from the human genome, codon optimized for expression in Saccharomyces cerevisiae. A HIS-tag was added at the beginning of the sequence on the extracellular side for detection purposes, chosen for its small size and the reduced likelihood that it would interfere in the receptor function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 748
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 76