Difference between revisions of "Part:BBa K2332030"
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<partinfo>BBa_K2332030 short</partinfo> | <partinfo>BBa_K2332030 short</partinfo> | ||
| − | This construct displays GFP fused with SpyTag on the cell surface of E. coli cells. Intimin' is a truncated version of Intimin (EaeA intimin) which has shown to display proteins of similar size to GFP fused to its N terminal. This construct allows to directly observe through fluorescence microscopy the functionality of Intimin for cell surface display. | + | This construct displays GFP fused with SpyTag on the cell surface of E. coli cells. Intimin' (159kDa) is a truncated version of Intimin (EaeA intimin) which has shown to display proteins of similar size to GFP fused to its N terminal. This construct allows to directly observe through fluorescence microscopy the functionality of Intimin for cell surface display. |
| − | + | It can be also used in combination with cells expressing our SpyCatcher construct variants to analyse their co-localization. SpyTag (13 amino acids) and SpyCatcher (138 amino acids, 15 kDa) are protein binding partners that originate from CnaB2 (immunoglobulin-llike collagen adhesin domain) of the FbaB protein, found in the invasive strains of S. pyogenes. | |
| − | + | ||
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Latest revision as of 23:38, 22 October 2017
Intimin'-GFP-SpyTag
This construct displays GFP fused with SpyTag on the cell surface of E. coli cells. Intimin' (159kDa) is a truncated version of Intimin (EaeA intimin) which has shown to display proteins of similar size to GFP fused to its N terminal. This construct allows to directly observe through fluorescence microscopy the functionality of Intimin for cell surface display. It can be also used in combination with cells expressing our SpyCatcher construct variants to analyse their co-localization. SpyTag (13 amino acids) and SpyCatcher (138 amino acids, 15 kDa) are protein binding partners that originate from CnaB2 (immunoglobulin-llike collagen adhesin domain) of the FbaB protein, found in the invasive strains of S. pyogenes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 801
Illegal NgoMIV site found at 1542 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2636