Difference between revisions of "Part:BBa K2305013"

Latest revision as of 22:46, 27 October 2017

pbad RBS Lacl

promoter pbad and LacI generator with RBS

Agarose gel electrophoresis

We calculated the new part’s base number to be 2389bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

Characterization map

Fig.The Growth curve of E.coil.

DNA sequencing

The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2383
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 4: / Line 4: @@
 promoter pbad and LacI generator with RBS
+==Agarose gel electrophoresis==
+We calculated the new part’s base number to be 2389bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
+[[Image:BIT Figure DNA sequencing K2305013.png|200px|left|K230500]]<br clear="all">
+==Characterization map==
+[[Image:BIT Figure map K2305013.png|400px|left|K230500]]<br clear="all">
+Fig.The Growth curve of E.coil.
+==DNA sequencing==
+The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further
+[[Image:BIT Figure DNA sequencing K2305013a.png|200px|left|K230500]][[Image:BIT Figure DNA sequencing K2305013b.png|200px|left|K230500]]<br clear="all">
 <!-- Add more about the biology of this part here