Difference between revisions of "Part:BBa K2514000:Design"

Latest revision as of 06:11, 19 October 2017

pSB1C3-miR-21-sponge-6s

Design Notes

Pervious studies indicated sponges containing bulged sites that are mispaired opposite miRNA are more effective than perfectly complementary sponges for individual microRNAs. And different miR-21 sponges contained different binding sites may have different accuracy for measuring the expression of miR-21 in colorectal cancer cells. So we desinged two different mir-21 sponges with different binding sits to test the efficiency of sponges. One mir-21 sponge contains six miR-21 binding sites with 3-nt spacers for bulged sites. We wanted to compare the accuracy and the effectiveness of different miR-21 sponges contained different binding sites for measuring the expression of miR-21 in colorectal cancer cells.

Source

We designed mir-21 sponges contains six miR-21 binding sites with 3-nt spacers for bulged sites based on the sequence of hsa-miR-21 according to the previous study (Ebert MS, Neilson JR, Sharp PA. MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat Methods. 2007 Sep;4(9):721-6)

References

Ebert MS, Neilson JR, Sharp PA. MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat Methods. 2007 Sep;4(9):721-6.

Ebert MS, Sharp PA. MicroRNA sponges: progress and possibilities. RNA. 2010 Nov;16(11):2043-50.