Difference between revisions of "Part:BBa K2443012"

Latest revision as of 23:30, 31 October 2017

LysRS optimized for expression in E. coli

Lysine tRNA synthetase responsible for attaching lysine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a N-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1436
Illegal NgoMIV site found at 1508
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 710

Revision as of 15:10, 18 October 2017 (view source) Graeme.glaister (Talk \| contribs) ← Older edit		Latest revision as of 23:30, 31 October 2017 (view source) Harlando (Talk \| contribs)
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	<partinfo>BBa_K2443012 short</partinfo>		<partinfo>BBa_K2443012 short</partinfo>

−	Lysine tRNA synthetase responsible for attaching lysine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal ~~histidine~~ tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.	+	Lysine tRNA synthetase responsible for attaching lysine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.