Difference between revisions of "Part:BBa K2209001"
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Q gene is the most important gene in aniline dioxygenase which had been confirmed relating with the degradation spectrum. Bacterial strain from different environment might have different degradation spectrum, compared with previous obtained strain, the combined gene (modified promoter and amplified Q gene) were expressed well. | Q gene is the most important gene in aniline dioxygenase which had been confirmed relating with the degradation spectrum. Bacterial strain from different environment might have different degradation spectrum, compared with previous obtained strain, the combined gene (modified promoter and amplified Q gene) were expressed well. | ||
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| − | === | + | === Characterization of part BBa_K2209001 === |
| + | Result showed as follows with well expression of the dioxygenase which have the potential of different degradation spectrum. | ||
| + | |||
| + | [[Image:pic 2.png|500px|left|thumb|]] | ||
| + | Lane M1: Protein marker<br> | ||
| + | Lane M2: Western blot marker<br> | ||
| + | Lane PC1: BSA (1 μg)<br> | ||
| + | Lane PC2: BSA (2 μg)<br> | ||
| + | Lane NC: Cell lysate without induction<br> | ||
| + | Lane 1: Cell lysate with induction for 16 h at 15 °C<br> | ||
| + | Lane 2: Cell lysate with induction for 4 h at 37 °C<br> | ||
| + | Lane NC1: Supernatant of cell lysate without induction<br> | ||
| + | Lane NC2: Pellet of cell lysate without induction<br> | ||
| + | Lane 3: Supernatant of cell lysate with induction for 16 h at 15 °C<br> | ||
| + | Lane 4: Pellet of cell lysate with induction for 16 h at 15 °C<br> | ||
| + | Lane 5: Supernatant of cell lysate with induction for 4 h at 37 °C<br> | ||
| + | Lane 6: Pellet of cell lysate with induction for 4 h at 37 °C<br> | ||
| + | |||
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Latest revision as of 07:20, 20 October 2017
Q gene of aniline dioxygenase from environmental samples
Q gene is the most important gene in aniline dioxygenase which had been confirmed relating with the degradation spectrum. Bacterial strain from different environment might have different degradation spectrum, compared with previous obtained strain, the combined gene (modified promoter and amplified Q gene) were expressed well.
Characterization of part BBa_K2209001
Result showed as follows with well expression of the dioxygenase which have the potential of different degradation spectrum.
Lane M1: Protein marker
Lane M2: Western blot marker
Lane PC1: BSA (1 μg)
Lane PC2: BSA (2 μg)
Lane NC: Cell lysate without induction
Lane 1: Cell lysate with induction for 16 h at 15 °C
Lane 2: Cell lysate with induction for 4 h at 37 °C
Lane NC1: Supernatant of cell lysate without induction
Lane NC2: Pellet of cell lysate without induction
Lane 3: Supernatant of cell lysate with induction for 16 h at 15 °C
Lane 4: Pellet of cell lysate with induction for 16 h at 15 °C
Lane 5: Supernatant of cell lysate with induction for 4 h at 37 °C
Lane 6: Pellet of cell lysate with induction for 4 h at 37 °C
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]