Difference between revisions of "Part:BBa K2365046"
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[[File: TEF1 TEF1-cyc1.jpeg|500px|center]] | [[File: TEF1 TEF1-cyc1.jpeg|500px|center]] | ||
| − | + | We used reserved restriction enzyme cutting site to inserted GPF (S6GT) between promoter and termiator as the index of promoter test. After 30 hours incubation, we measured the fluorescence intensity of the transformed yeast at 440 nm to 530 nm | |
| + | [[File: U-disk test.jpg|500px|center]] | ||
| + | [[File:酵母荧光.jpg|700px|center]] | ||
Latest revision as of 09:17, 1 November 2017
TEF1 promoter-CYC1 terminator
Between the TEF1 and cyc1,having the restriction enzyme cutting site.And you can insert the gene if you want.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 25
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 547
We used reserved restriction enzyme cutting site to inserted GPF (S6GT) between promoter and termiator as the index of promoter test. After 30 hours incubation, we measured the fluorescence intensity of the transformed yeast at 440 nm to 530 nm
