Difference between revisions of "Part:BBa K2232015"

 
 
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<partinfo>BBa_K2232015 short</partinfo>
 
<partinfo>BBa_K2232015 short</partinfo>
  
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promoter+coding protein
+
For facilitating the interconversion of CO2 and HCO3− which is beneficial to the precipitation of CaCO3, a plasmid containing Carbonic anhydrase coding gene have built and transferred into B. subtilis strain WB800 and express successfully.The promoter P43 used in the plasmid is a strong promoter in Bacillus subtilis 168,and the Carbonic anhydrase is from The Bacillus pseudofirmus OF4 (GenBank Acc.No. CP001878)which belong to α-type in classes of CAs. The terminator we used is rrnBT1(BBa_B0010) from E. coli rrnB, which will stop the Transcription of gene and raise the efficiency of expression.
  
 
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Latest revision as of 10:34, 22 October 2017


P43_OF4-CA_rrnBT1

For facilitating the interconversion of CO2 and HCO3− which is beneficial to the precipitation of CaCO3, a plasmid containing Carbonic anhydrase coding gene have built and transferred into B. subtilis strain WB800 and express successfully.The promoter P43 used in the plasmid is a strong promoter in Bacillus subtilis 168,and the Carbonic anhydrase is from The Bacillus pseudofirmus OF4 (GenBank Acc.No. CP001878)which belong to α-type in classes of CAs. The terminator we used is rrnBT1(BBa_B0010) from E. coli rrnB, which will stop the Transcription of gene and raise the efficiency of expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 879