Difference between revisions of "Part:BBa I742114"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_I742114 short</partinfo>
 
<partinfo>BBa_I742114 short</partinfo>
  
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gene is from Pseudomonas fluorescens  
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The gene is from ''Pseudomonas fluorescens''. The sequence was submitted to GeneArt for synthesis including codon optimisation (''Pseudomonas'' has a very high GC content whereas ''E. coli'' and ''Lactobacillus'' have much lower GC content).
  
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encodes feruoyl CoA synthase (ligates acetyl-CoA onto ferulic acid)  
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*encodes feruoyl CoA synthase (ligates acetyl-CoA onto ferulic acid)  
  
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produces feruloyl CoA from ferrulic acid
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*produces feruloyl CoA from ferulic acid
  
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catalyses the penultimate of the vanillin biosynthesis pathway  
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*catalyses the penultimate step of the vanillin biosynthesis pathway  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:59, 26 October 2007

Feruloyl-CoA synthase (fcs)

The gene is from Pseudomonas fluorescens. The sequence was submitted to GeneArt for synthesis including codon optimisation (Pseudomonas has a very high GC content whereas E. coli and Lactobacillus have much lower GC content).

  • encodes feruoyl CoA synthase (ligates acetyl-CoA onto ferulic acid)
  • produces feruloyl CoA from ferulic acid
  • catalyses the penultimate step of the vanillin biosynthesis pathway

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 30
    Illegal NgoMIV site found at 384
    Illegal NgoMIV site found at 1107
    Illegal AgeI site found at 535
    Illegal AgeI site found at 1072
    Illegal AgeI site found at 1533
  • 1000
    COMPATIBLE WITH RFC[1000]