Difference between revisions of "Part:BBa K2278022:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | A | + | A pAOX1 promoter is present on the pPICZalpha vector before the construction. It makes genome recombination easier in <i>Pichia pastoris</i> genome. |
Part:BBa_K1800001: Alpha-Factor Secretion Signal | Part:BBa_K1800001: Alpha-Factor Secretion Signal | ||
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===Source=== | ===Source=== | ||
− | The peptides DNA sequence has been obtained by reverse translate the amino acid sequence of the Leucrocin I proposed by Pata et al., 2011. | + | The peptides DNA sequence has been obtained by reverse translate the amino acid sequence of the Leucrocin I proposed by Pata <i>et al</i>., 2011. They had determinated the amino acid sequence by mass spectrometry analysis. |
===References=== | ===References=== | ||
Pata, S., Yaraksa, N., Daduang, S., Temsiripong, Y., Svasti, J., Araki, T. and Thammasirirak, S. (2011). Characterization of the novel antibacterial peptide Leucrocin from crocodile (Crocodylus siamensis) white blood cell extracts. Developmental & Comparative Immunology, 35(5), pp.545-553. | Pata, S., Yaraksa, N., Daduang, S., Temsiripong, Y., Svasti, J., Araki, T. and Thammasirirak, S. (2011). Characterization of the novel antibacterial peptide Leucrocin from crocodile (Crocodylus siamensis) white blood cell extracts. Developmental & Comparative Immunology, 35(5), pp.545-553. |
Latest revision as of 10:58, 30 October 2017
Lecrocin I antimicrobial peptide with Alpha-Factor Secretion Signal
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 244
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A pAOX1 promoter is present on the pPICZalpha vector before the construction. It makes genome recombination easier in Pichia pastoris genome. Part:BBa_K1800001: Alpha-Factor Secretion Signal
Source
The peptides DNA sequence has been obtained by reverse translate the amino acid sequence of the Leucrocin I proposed by Pata et al., 2011. They had determinated the amino acid sequence by mass spectrometry analysis.
References
Pata, S., Yaraksa, N., Daduang, S., Temsiripong, Y., Svasti, J., Araki, T. and Thammasirirak, S. (2011). Characterization of the novel antibacterial peptide Leucrocin from crocodile (Crocodylus siamensis) white blood cell extracts. Developmental & Comparative Immunology, 35(5), pp.545-553.