Difference between revisions of "Part:BBa K2278023:Design"
 
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===Design Notes===
 
===Design Notes===
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Part:BBa_K431009 : glyceraldehyde 3-phosphate dehydrogenase promoter P(GAP)
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A pAOX1 promoter is present on the pPICZalpha vector before the construction. It makes genome recombination easier in <i>Pichia pastoris</i> genome.
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Part:BBa_K1800001: Alpha-Factor Secretion Signal
 
Part:BBa_K1800001: Alpha-Factor Secretion Signal
  
 
===Source===
 
===Source===
  
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The peptides DNA sequence has been obtained by reverse translate the amino acid sequence of the proposed by Prajanban et al., 2017.
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The peptides DNA sequence has been obtained by reverse translate the amino acid sequence of the proposed by Prajanban <i>et al</i>., 2017.
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They had determinated the amino acid sequence by mass spectrometry analysis.  
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They had determinated the amino acid sequence by mass spectrometry analysis.  
  
  

Latest revision as of 11:02, 30 October 2017


cOT2 antimicrobial peptide with Alpha-Factor Secretion Signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A pAOX1 promoter is present on the pPICZalpha vector before the construction. It makes genome recombination easier in Pichia pastoris genome. Part:BBa_K1800001: Alpha-Factor Secretion Signal

Source

The peptides DNA sequence has been obtained by reverse translate the amino acid sequence of the proposed by Prajanban et al., 2017. They had determinated the amino acid sequence by mass spectrometry analysis.


References

Prajanban, B., Jangpromma, N., Araki, T. and Klaynongsruang, S. (2017). Antimicrobial effects of novel peptides cOT2 and sOT2 derived from Crocodylus siamensis and Pelodiscus sinensis ovotransferrins. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1859(5), pp.860-869. <p>Brogden, K. (2005). Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?. Nature Reviews Microbiology, 3(3), pp.238-250.