Difference between revisions of "Part:BBa I742110"

Latest revision as of 17:33, 25 October 2007

LIMS1

Limonene synthase 1 from Citrus limon (accession AF514287). Synthesised by Geneart. Limonene synthase converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent. Note: several codons were altered silently to remove several forbidden restriction sites. The N-terminal region (plastid targeting sequence) was removed, removing the first 51 amino acids and replacing codon 52 with the start codon ATG.

For a diagram of limonene synthesis, and the synthesis of other lemon flavour compounds see [http://2007.igem.org/Edinburgh/Yoghurt/Design#Lemon_Flavour_Production Edinburgh Yoghurt Wiki]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 100

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 __NOTOC__
 <partinfo>BBa_I742110 short</partinfo>
-Limonene synthase 1 from Citrus limon (accession AF514287)
+Limonene synthase 1 from ''Citrus limon'' (accession AF514287). Synthesised by Geneart. Limonene synthase converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent. '''Note:''' several codons were altered silently to remove several forbidden restriction sites. The N-terminal region (plastid targeting sequence) was removed, removing the first 51 amino acids and replacing codon 52 with the start codon ATG.
-Synthesised by Geneart.
+For a diagram of limonene synthesis, and the synthesis of other lemon flavour compounds see [http://2007.igem.org/Edinburgh/Yoghurt/Design#Lemon_Flavour_Production Edinburgh Yoghurt Wiki]
-Limonene synthase converts farnesyl-diphosphate to + limonene, which is a component of lemon scent.
 <!-- Add more about the biology of this part here