Difference between revisions of "Part:BBa K2353002"

Latest revision as of 16:04, 1 November 2017

tsPurpleLAA

tsPurpleLAA is composed of a ribosomal binding site, tsPurple (a purple chromoprotein which functions as a reporter), and LAA (a fast degradation tag). tsPurpleLAA is intended to be assembled with pLambdaR-LacI (which is the promoter and regulatory system) and r0011ClpXPCI (which is the non-lysosomal protease). The terminator is located at the beginning of pLac-ClpXP-CI. When fully assembled, p-lambda-r LacI transcribes tsPurpleLAA and LacI represses pLac, preventing transcription of ClpXP-CI. Upon induction with IPTG, LacI binds to IPTG which prevents repression of pLac. Therefore, ClpXP-CI is transcribed; ClpXP should recognize the LAA deg tag and degrade the tsPurple protein. Since LAA is a fast degradation tag, so tsPurpleLAA should have the highest levels of degradation seen. The ensuing levels of expression could then be compared to that of BBa_K2353000 (tsPurple without a deg tag) to see the relative level of degradation experienced.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2353002 short</partinfo>
-tsPurpleLAA is composed of a ribosomal binding site, tsPurple (a purple chromoprotein which functions as a reporter), and LAA, a fast degradation tag. tsPurpleLAA is intended to be assembled with pLambdaR-LacI (which is the promoter and regulatory system) and r0011ClpXPCI (which is the non-lysosomal protease).
+tsPurpleLAA is composed of a ribosomal binding site, tsPurple (a purple chromoprotein which functions as a reporter), and LAA (a fast degradation tag). tsPurpleLAA is intended to be assembled with pLambdaR-LacI (which is the promoter and regulatory system) and r0011ClpXPCI (which is the non-lysosomal protease). The terminator is located at the beginning of pLac-ClpXP-CI. When fully assembled, p-lambda-r LacI transcribes tsPurpleLAA and LacI represses pLac, preventing transcription of ClpXP-CI. Upon induction with IPTG, LacI binds to IPTG which prevents repression of pLac. Therefore, ClpXP-CI is transcribed; ClpXP should recognize the LAA deg tag and degrade the tsPurple protein. Since LAA is a fast degradation tag, so tsPurpleLAA should have the highest levels of degradation seen. The ensuing levels of expression could then be compared to that of BBa_K2353000 (tsPurple without a deg tag) to see the relative level of degradation experienced.
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