Difference between revisions of "Part:BBa K2325000"
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The BBa_K2325000 contains a coding sequence of formamidase which catalyzes the conversion of formamide to ammonia. | The BBa_K2325000 contains a coding sequence of formamidase which catalyzes the conversion of formamide to ammonia. | ||
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| + | <br /> | ||
| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K2325000 SequenceAndFeatures</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Formamidase is an enzyme involved in the production of ammonia for providing nitrogen source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of nitrogen in E.coli. | Formamidase is an enzyme involved in the production of ammonia for providing nitrogen source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of nitrogen in E.coli. | ||
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| + | <center><h4>HCO - <font color="red">NH<sub>2</sub></font> + H<sub>2</sub>O ⇒ HCOOH + <font color="red">NH<sub>3</sub></font></h4></center> | ||
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| + | ===Gel analysis=== | ||
| + | [[File:BBa_K2325000--1.png|300px]] | ||
===Enzymatic properties=== | ===Enzymatic properties=== | ||
| − | + | [[File:BBa_K2325000--2.png|thumb|left|600px|'''Figure 1''': Enzymatic activity of formamidase under different temperatures and pH]]<br /> | |
| − | < | + | |
| + | <br><table> | ||
| + | <tr><th> | ||
| + | [[File:BBa_K2325000--3.png|thumb|left|550px|'''Figure 2''': The Michaelis constant of formamidase]]</th><th> | ||
| + | [[File:BBa K2325000--5.png|thumb|right|330px|'''Figure 3''': Influence of metal ion on the enzymatic activity of formamidase]]<br /></th></tr></table> | ||
| + | [[File:BBa_K2325000--4.png|thumb|left|350px|]]<br /> | ||
Latest revision as of 15:07, 29 October 2017
Formamidase (FOR)
The BBa_K2325000 contains a coding sequence of formamidase which catalyzes the conversion of formamide to ammonia.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 69
Illegal NgoMIV site found at 556
Illegal NgoMIV site found at 751
Illegal NgoMIV site found at 898 - 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Formamidase is an enzyme involved in the production of ammonia for providing nitrogen source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of nitrogen in E.coli.
HCO - NH2 + H2O ⇒ HCOOH + NH3
Gel analysis
Enzymatic properties
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