Difference between revisions of "Part:BBa K2387003:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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This construct is used to determine the functionality of the araC/pBAD promoter. Furthermore it can be used as a positive control in many fluorescence based experiments.
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how you used this part and how it worked out.
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eYFP was expressed induced with different L-arabinose concentrations (0, 0.0002. 0.002 and 0.2%). Furthermore, sole eYFP termini were used as negative controls.
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Used protocol can be found at http://2017.igem.org/Team:Wageningen_UR/Notebook/Protocols
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<div class="figure-center-imagebox">
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                        https://static.igem.org/mediawiki/2017/9/9d/T--Wageningen_UR--araCpBAD_test.png"
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                    </div>
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We clearly show that increasing the concentration of L-arabinose leads to a higher fluorescent signal due to increased protein production.
  
 
===Applications of BBa_K2387003===
 
===Applications of BBa_K2387003===

Latest revision as of 07:48, 20 October 2017


This construct is used to determine the functionality of the araC/pBAD promoter. Furthermore it can be used as a positive control in many fluorescence based experiments.

eYFP was expressed induced with different L-arabinose concentrations (0, 0.0002. 0.002 and 0.2%). Furthermore, sole eYFP termini were used as negative controls.

Used protocol can be found at http://2017.igem.org/Team:Wageningen_UR/Notebook/Protocols 

                       T--Wageningen_UR--araCpBAD_test.png"

We clearly show that increasing the concentration of L-arabinose leads to a higher fluorescent signal due to increased protein production.

Applications of BBa_K2387003

User Reviews

UNIQ62cab2c86bac4f30-partinfo-00000000-QINU UNIQ62cab2c86bac4f30-partinfo-00000001-QINU