Difference between revisions of "Part:BBa K2368013"
 
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<h1>Introduction</h1>
 
<h1>Introduction</h1>
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<partinfo>BBa_K2368013 short</partinfo>
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<p style="text-align: center"><partinfo>BBa_K2368013 short</partinfo></P>
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<p> This part is the overlap of that sweetness receptor T1R3 combines with the YCP tag.  </p>
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<p> The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R3 and YCP tag.</P>
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<P> The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R3.</p>
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<h1>Design</h1>
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<p> Firstly, we constructed the specific primers that consists of the overlap regions of the T1R3 and YCP. Then, we combined the overlap of T1R3 with the YCP by PCR.</p>
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[[File:T-BIT-China-2017parts-17.png|center|500px|默认文字]]
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<p style="text-align: center">Fig.1 The schematic diagram of YCP+T1R3 overlap</p>
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[[File:T-BIT-China-2017parts-18.png|center|500px|默认文字]]
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<p style="text-align: center">Fig. 2 The PCR method of YCP+T1R3 overlap</p>
  
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<P>This part is that sweetness receptor T1R3 combines with the YCP tag. </P>
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<h1>Experiment</h1>
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<P>The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway is so important that it can’t be modified. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can only perform the fusion between the N-terminal of T1R3 and YCP tag.</P>
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<p>This part is used to detect the expression and the location of the T1R3.</p>
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<P>The result of the part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R3. </P>
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[[File:T-BIT-China-2017parts-19.png|center|500px|默认文字]]
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<p style="text-align: center">Fig.3 Electrophoresis of YCP+T1R3 overlap.Line 1 and 2 is the target. </p>
  
  

Latest revision as of 19:09, 26 October 2017


Introduction

T1R3-YCP

This part is the overlap of that sweetness receptor T1R3 combines with the YCP tag.

The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R3 and YCP tag.

The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R3.

Design

Firstly, we constructed the specific primers that consists of the overlap regions of the T1R3 and YCP. Then, we combined the overlap of T1R3 with the YCP by PCR.

默认文字

Fig.1 The schematic diagram of YCP+T1R3 overlap

默认文字

Fig. 2 The PCR method of YCP+T1R3 overlap

Experiment

This part is used to detect the expression and the location of the T1R3.

默认文字

Fig.3 Electrophoresis of YCP+T1R3 overlap.Line 1 and 2 is the target.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]