Difference between revisions of "Part:BBa K2246009:Experience"
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===Applications of BBa_K2246009=== | ===Applications of BBa_K2246009=== | ||
| + | For the following samples of BSLA devices (Blue chromoprotein, forward siRNA, loop and reverse siRNA): BSLA-AWD, BSLA-SOD, BSLA-WNT and BSLA-RACI the sequencing was made using the VF2 primer, although it was possible to confirm the presence of the blue chromoprotein, the reaction couldn´t show us the presence of the siRNAs constructs because of the length of the protein gene before them. A repetition of the sequencing using the VR primers for the BSLA-SOD and BSLA-RACI devices was performed to seek the siRNA construction before the blue chromoprotein, however the results weren´t conclusive since the alignment showed the correct base pairs just after the reverse primer but not the same sequence before the terminator. | ||
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| + | >Sequence obtained with the forward primer (VF2) | ||
| + | TTTTCTGCATTTGCCCGTGCCGCTCCATGGCCCACCCGTGAAGGTGAGCCCGTGAGTTGATTGCTACGTAATTAGTTAGTTAGCCCTTAGTGACTCGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAATACAAGCTACTTGTTCTTTTTGCATACTAGAGAAAGAGGAGAAATACTAGATGAGTGTGATCGCTAAACAAATGACCTACAAGGTTTATATGTCAGGCACGGTCAATGGACACTACTTTGAGGTCGAAGGCGATGGAAAAGGTAAGCCCTACGAGGGGGAGCAGACGGTAAAGCTCACTGTCACCAAGGGCGGACCTCTGCCATTTGCTTGGGATATTTTATCACCACAGTGTCAGTACGGAAGCATACCATTCACCAAGTACCCTGAAGACATCCCTGACTATGTAAAGCAGTCATTCCCGGAGGGCTATACATGGGAGAGGATCATGAACTTTGAAGATGGTGCAGTGTGTACTGTCAGCAATGATTCCAGCATCCAAGGCAACTGTTTCATCTACCATGTCAAGTCCTCTGGTTTGAACTTTCCTCCCAATGGACCTGTCATGCAGAAGAAGACACAGGGCTTGGA | ||
===User Reviews=== | ===User Reviews=== | ||
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<!-- Template for a user review | <!-- Template for a user review | ||
{|width='80%' style='border:1px solid gray' | {|width='80%' style='border:1px solid gray' | ||
| − | |- | + | |-For the following samples of BSLA devices (Blue chromoprotein, forward siRNA, loop and reverse siRNA): BSLA-AWD, BSLA-SOD, BSLA-WNT and BSLA-RACI the sequencing was made using the VF2 primer, although it was possible to confirm the presence of the blue chromoprotein, the reaction couldn´t show us the presence of the siRNAs constructs because of the length of the protein gene before them. A repetition of the sequencing using the VR primers for the BSLA-SOD and BSLA-RACI devices was performed to seek the siRNA construction before the blue chromoprotein, however the results weren´t conclusive since the alignment showed the correct base pairs just after the reverse primer but not the same sequence before the terminator. |
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| + | >Sequence obtained with the forward primer (VF2) | ||
| + | TTTTCTGCATTTGCCCGTGCCGCTCCATGGCCCACCCGTGAAGGTGAGCCCGTGAGTTGATTGCTACGTAATTAGTTAGTTAGCCCTTAGTGACTCGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAATACAAGCTACTTGTTCTTTTTGCATACTAGAGAAAGAGGAGAAATACTAGATGAGTGTGATCGCTAAACAAATGACCTACAAGGTTTATATGTCAGGCACGGTCAATGGACACTACTTTGAGGTCGAAGGCGATGGAAAAGGTAAGCCCTACGAGGGGGAGCAGACGGTAAAGCTCACTGTCACCAAGGGCGGACCTCTGCCATTTGCTTGGGATATTTTATCACCACAGTGTCAGTACGGAAGCATACCATTCACCAAGTACCCTGAAGACATCCCTGACTATGTAAAGCAGTCATTCCCGGAGGGCTATACATGGGAGAGGATCATGAACTTTGAAGATGGTGCAGTGTGTACTGTCAGCAATGATTCCAGCATCCAAGGCAACTGTTTCATCTACCATGTCAAGTCCTCTGGTTTGAACTTTCCTCCCAATGGACCTGTCATGCAGAAGAAGACACAGGGCTTGGA | ||
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|width='10%'| | |width='10%'| | ||
<partinfo>BBa_K2246009 AddReview number</partinfo> | <partinfo>BBa_K2246009 AddReview number</partinfo> | ||
Latest revision as of 03:54, 2 November 2017
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Applications of BBa_K2246009
For the following samples of BSLA devices (Blue chromoprotein, forward siRNA, loop and reverse siRNA): BSLA-AWD, BSLA-SOD, BSLA-WNT and BSLA-RACI the sequencing was made using the VF2 primer, although it was possible to confirm the presence of the blue chromoprotein, the reaction couldn´t show us the presence of the siRNAs constructs because of the length of the protein gene before them. A repetition of the sequencing using the VR primers for the BSLA-SOD and BSLA-RACI devices was performed to seek the siRNA construction before the blue chromoprotein, however the results weren´t conclusive since the alignment showed the correct base pairs just after the reverse primer but not the same sequence before the terminator.
>Sequence obtained with the forward primer (VF2) TTTTCTGCATTTGCCCGTGCCGCTCCATGGCCCACCCGTGAAGGTGAGCCCGTGAGTTGATTGCTACGTAATTAGTTAGTTAGCCCTTAGTGACTCGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAATACAAGCTACTTGTTCTTTTTGCATACTAGAGAAAGAGGAGAAATACTAGATGAGTGTGATCGCTAAACAAATGACCTACAAGGTTTATATGTCAGGCACGGTCAATGGACACTACTTTGAGGTCGAAGGCGATGGAAAAGGTAAGCCCTACGAGGGGGAGCAGACGGTAAAGCTCACTGTCACCAAGGGCGGACCTCTGCCATTTGCTTGGGATATTTTATCACCACAGTGTCAGTACGGAAGCATACCATTCACCAAGTACCCTGAAGACATCCCTGACTATGTAAAGCAGTCATTCCCGGAGGGCTATACATGGGAGAGGATCATGAACTTTGAAGATGGTGCAGTGTGTACTGTCAGCAATGATTCCAGCATCCAAGGCAACTGTTTCATCTACCATGTCAAGTCCTCTGGTTTGAACTTTCCTCCCAATGGACCTGTCATGCAGAAGAAGACACAGGGCTTGGA
User Reviews
UNIQd42b396a52e5180a-partinfo-00000000-QINU UNIQd42b396a52e5180a-partinfo-00000001-QINU