Difference between revisions of "Part:BBa K2324001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Codon optimisation of | + | Codon optimisation for expression in <i>E. coli </i>. Research was required to identify the ideal placement of the foreign protein domain. |
Latest revision as of 19:11, 16 October 2017
FimH+sfGFP at residue 225
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 185
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimisation for expression in E. coli . Research was required to identify the ideal placement of the foreign protein domain.
Source
The FimH sequence is a codon optimised version of the registry entry BBa_K1850001. The sfGFP sequence is a codon optimised version of the registry entry BBa_I746916.