Difference between revisions of "Part:BBa K2235005:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | 6xHis-tag is N terminally attached to the enzyme sequence. | |
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===Source=== | ===Source=== | ||
− | + | ||
===References=== | ===References=== | ||
+ | Egebjerg, J. and Christensen, S. (2005). Cloning, expression and characterization of a sialidase gene from Arthrobacter ureafaciens. Biotechnology and Applied Biochemistry, 41(3), p.225. |
Latest revision as of 19:44, 31 October 2017
Sialidase enzyme
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 55
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 502
Illegal NgoMIV site found at 577
Illegal NgoMIV site found at 667 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1047
Design Notes
6xHis-tag is N terminally attached to the enzyme sequence.
Source
References
Egebjerg, J. and Christensen, S. (2005). Cloning, expression and characterization of a sialidase gene from Arthrobacter ureafaciens. Biotechnology and Applied Biochemistry, 41(3), p.225.