Difference between revisions of "Part:BBa K2324001:Design"

 
 
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===Design Notes===
 
===Design Notes===
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Codon optimisation of CDS.
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Codon optimisation for expression in <i>E. coli </i>. Research was required to identify the ideal placement of the foreign protein domain.  
  
  
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===Source===
 
===Source===
  
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All the components of this part are from the iGEM registry originally, but the CDS is codon optimised for expression in <i>E.coli</i> . The FimH sequence we used is part BBa_K1850001, the T7 promoter source was *INSERT HERE* and the GFP sequence was from part BBa_I746916.
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The FimH sequence is a codon optimised version of the registry entry BBa_K1850001. The sfGFP sequence is a codon optimised version of the registry entry BBa_I746916.
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===References===
 
===References===

Latest revision as of 19:11, 16 October 2017


FimH+sfGFP at residue 225


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation for expression in E. coli . Research was required to identify the ideal placement of the foreign protein domain.


Source

The FimH sequence is a codon optimised version of the registry entry BBa_K1850001. The sfGFP sequence is a codon optimised version of the registry entry BBa_I746916.

References