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===Applications of BBa_K1900003===
 
===Applications of BBa_K1900003===
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This part works to supplant the antibiotic efflux function of a deletion strain of E. coli that lacks the gene. The deletion strain shows little resistance to novobiocin and erythromycin but is able to resist both of these when transformed with the tolc gene. Below a comparison is made between the strain lacking the tolc gene, and the strain with the E. coli tolc gene. A Kirby Bauer assay was done to assess efflux function. The larger the zone of inhibition, the less resistant the bacteria is to the antibiotic.  Additionally, the E. coli tolc gene was compared to other tolc genes from various gram negative bacteria. These include Serratia marcescens and Klebsiella pneumonia. A 3D model of the protein is also shown below.
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|}; This part works to supplant the antibiotic efflux function of a deletion strain of E. coli that lacks the gene. The deletion strain shows little resistance to novobiocin and erythromycin, but is able to resist both of these when transformed with the tolc gene.
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Latest revision as of 21:55, 9 October 2017


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1900003

This part works to supplant the antibiotic efflux function of a deletion strain of E. coli that lacks the gene. The deletion strain shows little resistance to novobiocin and erythromycin but is able to resist both of these when transformed with the tolc gene. Below a comparison is made between the strain lacking the tolc gene, and the strain with the E. coli tolc gene. A Kirby Bauer assay was done to assess efflux function. The larger the zone of inhibition, the less resistant the bacteria is to the antibiotic. Additionally, the E. coli tolc gene was compared to other tolc genes from various gram negative bacteria. These include Serratia marcescens and Klebsiella pneumonia. A 3D model of the protein is also shown below.

User Reviews

UNIQ52463e0211be14ed-partinfo-00000000-QINU UNIQ52463e0211be14ed-partinfo-00000001-QINU