Difference between revisions of "Part:BBa K2308006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | There's a point mutant in the sequence in order to avoid specific | + | There's a point mutant in the sequence in order to avoid specific restriction site. |
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===Source=== | ===Source=== | ||
Latest revision as of 06:55, 5 October 2017
upstream sequence of the gene crtB in genome of Rhodobacter Sphaeroides
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 93
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 47
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 162
Design Notes
There's a point mutant in the sequence in order to avoid specific restriction site.
Source
from the genome of Rhodobacter Sphaeroides