Difference between revisions of "Part:BBa K2443000"

Latest revision as of 23:07, 31 October 2017

AlaRS optimized for expression in E. coli

Alanine tRNA synthetase responsible for attaching alanine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 513
Illegal BglII site found at 720
Illegal BamHI site found at 686
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1124
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2524
Illegal BsaI site found at 2701
Illegal BsaI.rc site found at 1073
Illegal SapI site found at 1808

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2443000 short</partinfo>
-Alanine tRNA synthetase responsible for attaching alanine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.
+Alanine tRNA synthetase responsible for attaching alanine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.
@@ Line 11: / Line 11: @@
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K2443003 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K2443000 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
-<partinfo>BBa_K2443003 parameters</partinfo>
+<partinfo>BBa_K2443000 parameters</partinfo>
 <!-- -->