Difference between revisions of "Part:BBa K2365031"
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TDH1 Yeast promoter | TDH1 Yeast promoter | ||
| − | Constitutive expression | + | Constitutive expression ; |
| − | + | Glyceraldehyde-3-phosphate dehydrogenase,a weak constitutive promoter,which catalyzed 3-phosphoglyceraldehyde oxidation (dehydrogenation) and phosphorylation.As the center of glucose metabolism,TDH1 plays an important role in glucose metabolism. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<partinfo>BBa_K2365031 parameters</partinfo> | <partinfo>BBa_K2365031 parameters</partinfo> | ||
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| + | ====PCR result==== | ||
| + | [[File:TDH1 TDH1-cyc1 NAU-04.jpeg|300px|center]] | ||
| + | [[File:启动子全部.jpeg|700px|center]] | ||
| + | Figure.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h. | ||
| + | Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available. | ||
Latest revision as of 01:25, 2 November 2017
TDH1 promotor
TDH1 Yeast promoter Constitutive expression ; Glyceraldehyde-3-phosphate dehydrogenase,a weak constitutive promoter,which catalyzed 3-phosphoglyceraldehyde oxidation (dehydrogenation) and phosphorylation.As the center of glucose metabolism,TDH1 plays an important role in glucose metabolism. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 431
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
PCR result
Figure.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h. Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available.