Difference between revisions of "Part:BBa K2447000:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
Used a stronger RBS (RBS 34) to elicit much stronger expression of GFP as compared to original system that was proposed by iGEM08_NYMU-Taipei which ultilised a weaker RBS (RBS 32) for GFP expression (https://parts.igem.org/Part:BBa_K116404)
+
Used a stronger RBS (RBS BBa_B0034) to elicit much stronger expression of GFP as compared to original system that was proposed by iGEM08_NYMU-Taipei which ultilised a weaker RBS (RBS BBa_B0032) for GFP expression (https://parts.igem.org/Part:BBa_K116404)
  
  

Latest revision as of 01:31, 25 October 2017


Extracellular phosphate sensor with GFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1162


Design Notes

Used a stronger RBS (RBS BBa_B0034) to elicit much stronger expression of GFP as compared to original system that was proposed by iGEM08_NYMU-Taipei which ultilised a weaker RBS (RBS BBa_B0032) for GFP expression (https://parts.igem.org/Part:BBa_K116404)


Source

From the iGEM parts registry

References