Difference between revisions of "Part:BBa K2259081"

 
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<partinfo>BBa_K2259081 short</partinfo>
 
<partinfo>BBa_K2259081 short</partinfo>
  
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This backbone is used to create a multiple-plasmid system. It can be used for general purposes as well.
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This is the updated version of iGEM base vector [[Part:BBa_I51020]] that has been withdrawn from the database due to encoding a ccdB toxin. Compared to BBa_I51020 the ccdB toxin was switched out to blue and white selection for picking colonies with the replaced origin of replication. [[Part:BBa_I51020]] page can still be used as a reliable source for this part's design and function.
  
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2259081 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2259081 SequenceAndFeatures</partinfo>
  
  
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===About SynORI===
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===Functional Parameters===
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[[Image:synori.png|600px|center|]]
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<partinfo>BBa_K2259081 parameters</partinfo>
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SynORI is a framework for multi-plasmid systems created by ''Vilnius-Lithuania 2017'' which enables quick and easy workflow with multiple plasmids, while also allowing to freely pick and modulate copy number for every unique plasmid group! Read more about [http://2017.igem.org/Team:Vilnius-Lithuania SynORI here]!
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==Base vector 2.0 in SynORI framework==
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[[File:collect.png|950px]]
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Engineering an improved, functional base vector 2.0 is crucial for the SynORI framework.
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Building a modular a synthetic origin of replication is easiest when working in biobrick region. But for synthetic ORI this is only possible if there is no other origin of replication in the rest of the vector. Base vector 2.0 provides this, as it has its pUC replicon in biobrick site.
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One can then replace the pUC origin of replication to SynORI system parts. Once the modular SynORI system is built, it can be transferred to another plasmid location and biobricks are then free to use for any task.
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See [http://2017.igem.org/Team:Vilnius-Lithuania Vilnius-Lithuania 2017 team wiki] for more insight about our synthetic origin of replication (SynORI).
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=Characterization of RNA II (Vilnius-Lithuania 2017)=
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==RNA I inactivation in wild type replicon==
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==References==
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<references />

Latest revision as of 12:44, 1 November 2017


Base vector 2.0 - a backbone for SynORI multi-plasmid framework

This is the updated version of iGEM base vector Part:BBa_I51020 that has been withdrawn from the database due to encoding a ccdB toxin. Compared to BBa_I51020 the ccdB toxin was switched out to blue and white selection for picking colonies with the replaced origin of replication. Part:BBa_I51020 page can still be used as a reliable source for this part's design and function.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 968
    Illegal suffix found at 2107
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 968
    Illegal NheI site found at 745
    Illegal NheI site found at 2283
    Illegal SpeI site found at 2108
    Illegal PstI site found at 2122
    Illegal NotI site found at 974
    Illegal NotI site found at 2115
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 968
    Illegal BglII site found at 1451
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 968
    Illegal suffix found at 2108
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 968
    Plasmid lacks a suffix.
    Illegal XbaI site found at 983
    Illegal SpeI site found at 2108
    Illegal PstI site found at 2122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


About SynORI

Synori.png

SynORI is a framework for multi-plasmid systems created by Vilnius-Lithuania 2017 which enables quick and easy workflow with multiple plasmids, while also allowing to freely pick and modulate copy number for every unique plasmid group! Read more about [http://2017.igem.org/Team:Vilnius-Lithuania SynORI here]!

Base vector 2.0 in SynORI framework

Collect.png


Engineering an improved, functional base vector 2.0 is crucial for the SynORI framework.

Building a modular a synthetic origin of replication is easiest when working in biobrick region. But for synthetic ORI this is only possible if there is no other origin of replication in the rest of the vector. Base vector 2.0 provides this, as it has its pUC replicon in biobrick site.

One can then replace the pUC origin of replication to SynORI system parts. Once the modular SynORI system is built, it can be transferred to another plasmid location and biobricks are then free to use for any task.


See [http://2017.igem.org/Team:Vilnius-Lithuania Vilnius-Lithuania 2017 team wiki] for more insight about our synthetic origin of replication (SynORI).

Characterization of RNA II (Vilnius-Lithuania 2017)

RNA I inactivation in wild type replicon

References