Difference between revisions of "Part:BBa K2239007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | ||
| + | The CBD tag is added to purify the effective enzymes. When connected with the enzyme DNA and expressed, CBD is able to bind to the cellulose on gauze. After washing off other chemicals in the solution, the enzymes are left on the gauze. As a result, they are successfully immobilized. | ||
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===Source=== | ===Source=== | ||
| − | + | The 7beta-HSDH DNA is originally from the genome of R. torques ATCC 35915, and is artificially synthesized based on the sequence retrieved from the GenBank. | |
| − | CBD | + | |
| + | The CBD sequence is retrieved from the GenBank and artificially synthesized. | ||
| + | |||
===References=== | ===References=== | ||
| + | [1] Ming-Min Zheng, Ru-Feng Wang, Chun-Xiu Li, Jian-He Xu: Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques. Process Biochemistry, Elsevier, 2015. | ||
| + | |||
| + | [2] Etai Shpigel, Arie Goldlust, Gilat Efroni, Amos Avraham, Adi Eshel, Mara Dekel, Oded Shoseyov: Immobilization of Recombinant Heparinase I Fused to Cellulose-Binding Domain, 1999. | ||
Latest revision as of 02:15, 1 November 2017
CBD-7betaHSDH
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1122
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 537
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 978
Design Notes
The CBD tag is added to purify the effective enzymes. When connected with the enzyme DNA and expressed, CBD is able to bind to the cellulose on gauze. After washing off other chemicals in the solution, the enzymes are left on the gauze. As a result, they are successfully immobilized.
Source
The 7beta-HSDH DNA is originally from the genome of R. torques ATCC 35915, and is artificially synthesized based on the sequence retrieved from the GenBank.
The CBD sequence is retrieved from the GenBank and artificially synthesized.
References
[1] Ming-Min Zheng, Ru-Feng Wang, Chun-Xiu Li, Jian-He Xu: Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques. Process Biochemistry, Elsevier, 2015.
[2] Etai Shpigel, Arie Goldlust, Gilat Efroni, Amos Avraham, Adi Eshel, Mara Dekel, Oded Shoseyov: Immobilization of Recombinant Heparinase I Fused to Cellulose-Binding Domain, 1999.