Difference between revisions of "Part:BBa K2379011:Design"

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===Design Notes===
 
===Design Notes===
  
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The circuit is under the regulation of the pLAC promoter. When inserted in a high copy number plasmid pLAC behaves as a constitutive promoter and hence does not require IPTG induction. However, if inserted in a low copy number plasmid, the system will have to be induced with IPTG as it would then behave like an inducible promoter. GFP, in this case, will be produced only in the presence of IPTG and at pH 8.4
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The circuit is under the regulation of the pLAC promoter. When inserted in a high copy number plasmid pLAC behaves as a constitutive promoter and hence does not require IPTG induction. However, if inserted in a low copy number plasmid, the system will have to be induced with IPTG as it would then behave like an inducible promoter. GFP, in this case, will be produced only in the presence of IPTG and at pH 8.5
  
 
===Source===
 
===Source===
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===References===
 
===References===
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1. Chang, C. L.,Lei Qi, Lucks J.B., Segall-Shapiro T.H., Wang, D.,Mutalik V.K.&  Arkin, A.P.An adaptor from translational to transcriptional control enables predictable assembly of complex regulation. Nat. Methods 9,1088–1094(2012).
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2. Nechooshtan, G., Elgrably-Weiss, M., Sheaffer, A.,Westhof, E., & Altuvia, S. A pH-responsive riboregulator.Genes Dev. 23, 2650–2662 (2009).

Latest revision as of 20:45, 1 November 2017


Adaptor with RNA pH meter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 181
    Illegal BsaI.rc site found at 1219


Design Notes

The circuit is under the regulation of the pLAC promoter. When inserted in a high copy number plasmid pLAC behaves as a constitutive promoter and hence does not require IPTG induction. However, if inserted in a low copy number plasmid, the system will have to be induced with IPTG as it would then behave like an inducible promoter. GFP, in this case, will be produced only in the presence of IPTG and at pH 8.5

Source

iGEM Registry

References

1. Chang, C. L.,Lei Qi, Lucks J.B., Segall-Shapiro T.H., Wang, D.,Mutalik V.K.& Arkin, A.P.An adaptor from translational to transcriptional control enables predictable assembly of complex regulation. Nat. Methods 9,1088–1094(2012).

2. Nechooshtan, G., Elgrably-Weiss, M., Sheaffer, A.,Westhof, E., & Altuvia, S. A pH-responsive riboregulator.Genes Dev. 23, 2650–2662 (2009).