Difference between revisions of "Part:BBa K2379008"
 
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<partinfo>BBa_K2379008 short</partinfo>
 
<partinfo>BBa_K2379008 short</partinfo>
  
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Uses the coding sequence of the tnaC peptide(BBa_K2379000) followed by BBa_K2379001 which consists of the Rho dependent terminator region followed by a RBS. Converts translational regulation of a riboswitch to transcriptional regulation
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This construct consists of the coding sequence of the tnaC leader peptide [https://parts.igem.org/Part:BBa_K2379000#BBa_K2379000 BBa_K2379000](BBa_K2379000) followed by the Rho-dependent terminator region [https://parts.igem.org/Part:BBa_K2379001#BBa_K2379001 BBa_K2379001](BBa_K2379001). It converts the translational regulatory property of a riboswitch to transcriptional regulation
  
 
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<p>
 
===Usage and Biology===
 
===Usage and Biology===
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The adaptor was characterised using the following three parts:
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1) Adaptor with constitutive RBS:
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https://static.igem.org/mediawiki/2017/8/84/T--SVCE_CHENNAI--result_graph_6.jpg
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Further characterisation can be found in the link provided below:
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[https://parts.igem.org/Part:BBa_K2379009#BBa_K2379009 BBa_K2379009](BBa_K2379009)
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2) Adaptor with FourU RNA Thermometer:
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https://static.igem.org/mediawiki/2017/8/85/T--SVCE_CHENNAI--result_graph_5.jpg
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Further characterisation can be found in the link provided below:
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[https://parts.igem.org/Part:BBa_K2379010#BBa_K2379010 BBa_K2379010](BBa_K2379010)
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3) Adaptor with RNA pHMeter:
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https://static.igem.org/mediawiki/2017/b/b6/T--SVCE_CHENNAI--result_graph_3.jpg
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Further characterisation can be found in the link provided below:
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[https://parts.igem.org/Part:BBa_K2379011#BBa_K2379011 BBa_K2379011](BBa_K2379011)
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</p>
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Latest revision as of 03:30, 2 November 2017


Adaptor

This construct consists of the coding sequence of the tnaC leader peptide BBa_K2379000(BBa_K2379000) followed by the Rho-dependent terminator region BBa_K2379001(BBa_K2379001). It converts the translational regulatory property of a riboswitch to transcriptional regulation

Usage and Biology

The adaptor was characterised using the following three parts:

1) Adaptor with constitutive RBS:

T--SVCE_CHENNAI--result_graph_6.jpg

Further characterisation can be found in the link provided below:

BBa_K2379009(BBa_K2379009)

2) Adaptor with FourU RNA Thermometer:


T--SVCE_CHENNAI--result_graph_5.jpg

Further characterisation can be found in the link provided below:

BBa_K2379010(BBa_K2379010)

3) Adaptor with RNA pHMeter:

T--SVCE_CHENNAI--result_graph_3.jpg

Further characterisation can be found in the link provided below:

BBa_K2379011(BBa_K2379011)



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]