Difference between revisions of "Part:BBa K2379007:Design"

 
 
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===Design Notes===
 
===Design Notes===
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None
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The circuit is under the regulation of the pLAC promoter. When inserted in a high copy number plasmid pLAC behaves as a constitutive promoter and hence does not require IPTG induction. However, if inserted in a low copy number plasmid, the system will have to be induced with IPTG as it would then behave like an inducible promoter. GFP, in this case, will be produced only in the presence of IPTG and at pH 8.4
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===Source===
 
===Source===
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===References===
 
===References===
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1.Nechooshtan, G., Elgrably-Weiss, M., Sheaffer, A.,Westhof, E., & Altuvia, S. A pH-responsive riboregulator.Genes Dev. 23, 2650–2662 (2009).
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2.Nechooshtan, G., Elgrably-Weiss, M., & Altuvia, S.Changes in transcriptional pausing modify the folding dynamics of the pH-responsive RNA element. Nucleic Acids research. 42(1), 622-630 (2013)
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3.Bingham, R., Karen, H., Joan S. Alkaline induction of a Novel Gene Locus, alx, in Escherichia coli. Journal of Bacteriology. 172(4), 2184-2186(1990)

Latest revision as of 15:19, 1 November 2017


pH sensitive system with RNA pHmeter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 181
    Illegal BsaI.rc site found at 916


Design Notes

The circuit is under the regulation of the pLAC promoter. When inserted in a high copy number plasmid pLAC behaves as a constitutive promoter and hence does not require IPTG induction. However, if inserted in a low copy number plasmid, the system will have to be induced with IPTG as it would then behave like an inducible promoter. GFP, in this case, will be produced only in the presence of IPTG and at pH 8.4

Source

iGEM Registry

References

1.Nechooshtan, G., Elgrably-Weiss, M., Sheaffer, A.,Westhof, E., & Altuvia, S. A pH-responsive riboregulator.Genes Dev. 23, 2650–2662 (2009).

2.Nechooshtan, G., Elgrably-Weiss, M., & Altuvia, S.Changes in transcriptional pausing modify the folding dynamics of the pH-responsive RNA element. Nucleic Acids research. 42(1), 622-630 (2013)

3.Bingham, R., Karen, H., Joan S. Alkaline induction of a Novel Gene Locus, alx, in Escherichia coli. Journal of Bacteriology. 172(4), 2184-2186(1990)