Difference between revisions of "Part:BBa K2379004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This part has been obtained by replacing the Shine Dalgarno sequence of E coli to that of Bacillus Subtilis, present in the RNA Thermometer[https://parts.igem.org/Part:BBa_K2379001#BBa_K2379001 BBa_K2379001](BBa_K2379001). The sequence was fed to RNA VIENNA FOLD and it was found that the structure of the RNA thermometer for Bacillus Subtilis was very similar to that of E Coli with nearly equal thermodynamic free energies. | |
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===Source=== | ===Source=== | ||
| − | + | nil | |
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===References=== | ===References=== | ||
| + | 1. Band, L. and Henner,D. J.: Bacillus subtilis requires a “stringent” Shine-Dalgarno region for gene expression.DNA 3 (1984) 17-21. | ||
Latest revision as of 03:17, 2 November 2017
RNA Thermometer Optimised for Bacillus Subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part has been obtained by replacing the Shine Dalgarno sequence of E coli to that of Bacillus Subtilis, present in the RNA ThermometerBBa_K2379001(BBa_K2379001). The sequence was fed to RNA VIENNA FOLD and it was found that the structure of the RNA thermometer for Bacillus Subtilis was very similar to that of E Coli with nearly equal thermodynamic free energies.
Source
nil
References
1. Band, L. and Henner,D. J.: Bacillus subtilis requires a “stringent” Shine-Dalgarno region for gene expression.DNA 3 (1984) 17-21.