Difference between revisions of "Part:BBa K2215002"

Latest revision as of 05:59, 1 October 2017

FeSOD Clade C

Superoxide dismutases (SOD) are the first line of defense against reactive oxygen species when cells become stressed. This makes them important antioxidants in the cell that is responsible for converting superoxide anion radicals into hydrogen peroxide, which can be further converted into water. (Alscher et al., 2002) There are various types of SODs, but this one is known as iron superoxide dismutase (FeSOD), which resides in the chloroplast. The sequence for this gene came from a heat resistant Clade C Symbiodinium, pulled from NCBI. This FeSOD is characterized in Chlamydomonas and therefore should be utilized in Chlamydomonas or it can be codon optimized for other species.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 130
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 166

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 <partinfo>BBa_K2215002 short</partinfo>
-There are different types of FeSODs from Symbiodinium, but this SOD came from Clade C, which is one of the most heat resistant clades of Symbiodinium.
+Superoxide dismutases (SOD) are the first line of defense against reactive oxygen species when cells become stressed. This makes them important antioxidants in the cell that is responsible for converting superoxide anion radicals into hydrogen peroxide, which can be further converted into water. (Alscher et al., 2002)
-FeSOD resides in the chloroplast, whose main function is to convert superoxide anion radicals into hydrogen peroxide that can later be converted into water. This FeSOD is characterized in Chlamydomonas.
+There are various types of SODs, but this one is known as iron superoxide dismutase (FeSOD), which resides in the chloroplast.
+The sequence for this gene came from a heat resistant Clade C Symbiodinium, pulled from NCBI.
+This FeSOD is characterized in Chlamydomonas and therefore should be utilized in Chlamydomonas or it can be codon optimized for other species.
 <!-- Add more about the biology of this part here