Difference between revisions of "Part:BBa K2332003:Design"

 
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===Source===
 
===Source===
  
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Obtained as a synthetic gBlock
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tRNA-Pyl (pyIT) sequence was obtained from: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1223014 BBa_K1223014]
  
 
===References===
 
===References===
 +
1. Mitra N. Incorporating Unnatural Amino Acids into Recombinant Proteins in Living Cells. Materials and Methods. 2013;3.
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 +
2. Wang Y, Wu B, Wang Z, Huang Y, Wan W, Russell W et al. A genetically encoded photocaged Nε-methyl-l-lysine. Molecular BioSystems. 2010;6(9):1557.

Latest revision as of 19:09, 23 October 2017


tRNA-Pyl (pylT)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

E. coli cells must be amberless in order to test the pyrrolysine incorporation only in the desired proteins.


Source

tRNA-Pyl (pyIT) sequence was obtained from: BBa_K1223014

References

1. Mitra N. Incorporating Unnatural Amino Acids into Recombinant Proteins in Living Cells. Materials and Methods. 2013;3.

2. Wang Y, Wu B, Wang Z, Huang Y, Wan W, Russell W et al. A genetically encoded photocaged Nε-methyl-l-lysine. Molecular BioSystems. 2010;6(9):1557.