Difference between revisions of "Part:BBa K2332002:Design"

 
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===Design Notes===
 
===Design Notes===
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We created several constructs comprised of this promoter and reporters. We then have to fuse the EL222 coding gene downstream of each reporter to test blue light induction.
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Designed by Jayaraman P. et al. (2016)
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===Source===
 
===Source===
  
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Obtained as a synthetic gBlock
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The DNA sequence was synthesised by Integrated DNA Technologies (IDT)
  
 
===References===
 
===References===
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Jayaraman P, Devarajan K, Chua T, Zhang H, Gunawan E, Poh C. Blue light-mediated transcriptional activation and repression of gene expression in bacteria. Nucleic Acids Research. 2016;44(14):6994-7005.

Latest revision as of 21:10, 22 October 2017


Blue light inducible promoter (Pblind)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed by Jayaraman P. et al. (2016)

Source

The DNA sequence was synthesised by Integrated DNA Technologies (IDT)

References

Jayaraman P, Devarajan K, Chua T, Zhang H, Gunawan E, Poh C. Blue light-mediated transcriptional activation and repression of gene expression in bacteria. Nucleic Acids Research. 2016;44(14):6994-7005.