Difference between revisions of "Part:BBa K2235003:Design"

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===Design Notes===
 
===Design Notes===
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There is a multiple cloning site(BamHI, SalI, KpnI, NsiI) between the two expression cassettes. Moreover, XmaI and KasI restriction sites can be found before whereas NdeI and MfeI after the BFP enabling any user to switch to the gene of interest.
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There is a multiple cloning site (BamHI, SalI, KpnI, NsiI) downstream of the double terminator B0014 and upstream of the K875001. Moreover, XmaI and KasI restriction sites can be found before the tagBFP whereas NdeI and MfeI after, enabling further subcloning of any gene of interest.
  
 
===Source===
 
===Source===

Latest revision as of 13:11, 23 October 2017


Cumate gene switch expressing BFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 418
    Illegal XhoI site found at 199
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101


Design Notes

There is a multiple cloning site (BamHI, SalI, KpnI, NsiI) downstream of the double terminator B0014 and upstream of the K875001. Moreover, XmaI and KasI restriction sites can be found before the tagBFP whereas NdeI and MfeI after, enabling further subcloning of any gene of interest.

Source

Obtained as G-block from IDT and amplified via PCR

References