Difference between revisions of "Part:BBa K2255010"
 
(2 intermediate revisions by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2255010 short</partinfo>
 
<partinfo>BBa_K2255010 short</partinfo>
  
−
This part is sequence coding for the D1 and D2 of the protein 3 of Pf3, a filamentous phage that target P.aeruginosa. Those domains are required for phage adsorption and entry.
+
This part is sequence coding for the D1 and D2 of the protein 3 of Pf3, a filamentous phage that target ''P. aeruginosa''. Those domains are required for phage adsorption and entry.
  
 
This part was design with Freiburg ([https://parts.igem.org/Assembly_standard_25 Rfc25]) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.  
 
This part was design with Freiburg ([https://parts.igem.org/Assembly_standard_25 Rfc25]) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.  
Line 9: Line 9:
 
This part is related to our collection part of filamentous phages attachment protein. To see all the part from this collection, you can check out the first part of this collection : [https://parts.igem.org/Part:BBa_K2255008 BBa_K2255008]
 
This part is related to our collection part of filamentous phages attachment protein. To see all the part from this collection, you can check out the first part of this collection : [https://parts.igem.org/Part:BBa_K2255008 BBa_K2255008]
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
This biobrick will be use to engineer phage-like particles targetting ''Pseudomonas aeruginosa''. Check out our [http://2017.igem.org/Team:Aix-Marseille/M13_Design design page] to know more about their design.
  
 
<!-- -->
 
<!-- -->

Latest revision as of 02:58, 2 November 2017


p3_P.aeruginosa (Rfc25)

This part is sequence coding for the D1 and D2 of the protein 3 of Pf3, a filamentous phage that target P. aeruginosa. Those domains are required for phage adsorption and entry.

This part was design with Freiburg (Rfc25) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.

This part is related to our collection part of filamentous phages attachment protein. To see all the part from this collection, you can check out the first part of this collection : BBa_K2255008

Usage and Biology

This biobrick will be use to engineer phage-like particles targetting Pseudomonas aeruginosa. Check out our [http://2017.igem.org/Team:Aix-Marseille/M13_Design design page] to know more about their design.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 433
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 814