Difference between revisions of "Part:BBa K2309022:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The purpose of coupling 6xHis-tag with LL-37can also be used for purifying the anti-microbial peptide produced in the bacterial cells.6xHis tag is six histidine amino acids tandem arranged together which has a strong affinity with the nickel ion in the Ni-NTA purification system. After purification, immunodot blot method was used to confirm that LL-37 was produced. | |
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===Source=== | ===Source=== | ||
Latest revision as of 07:54, 1 November 2017
LL-37 with A His-Tag for Lactococcus lactis NZ9000 (codon optimized)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The purpose of coupling 6xHis-tag with LL-37can also be used for purifying the anti-microbial peptide produced in the bacterial cells.6xHis tag is six histidine amino acids tandem arranged together which has a strong affinity with the nickel ion in the Ni-NTA purification system. After purification, immunodot blot method was used to confirm that LL-37 was produced.
Source
Homo sapiens