Difference between revisions of "Part:BBa K2305010"

 
 
(3 intermediate revisions by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2305010 short</partinfo>
 
<partinfo>BBa_K2305010 short</partinfo>
  
−
It's a GFP/RFP dual fluorescent system,
+
It's a GFP/RFP dual fluorescent system
 +
==Agarose gel electrophoresis==
 +
We calculated the new part’s base number to be 3134bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
 +
[[Image:BIT Figure DNA sequencing K2305010.png|200px|left|K230500]]<br clear="all">
 +
 
 +
DNA sequencing[edit]
 +
The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further
 +
[[Image:BIT Figure DNA sequencing K2305010a.png|200px|left|K230500]]
 +
[[Image:BIT Figure DNA sequencing K2305010b.png|200px|left|K230500]]
 +
<br clear="all">
 +
==Characterization map==
 +
[[Image:BIT Figure map K2305010.png|400px|left|K230500]]<br clear="all">
 +
Fig.The Growth curve of E.coil.
 +
[[Image:BIT Figure map K2305010a.png|400px|left|K230500]]<br clear="all">
 +
Fig.The GFP curve of E.coil.
 +
[[Image:BIT Figure map K2305010b.png|400px|left|K230500]]<br clear="all">
 +
Fig.The RFP curve of E.coil.
 +
 
 +
 
 +
 
  
  

Latest revision as of 11:47, 29 October 2017


GFP/RFP dual fluorescent system with LacI and plac

It's a GFP/RFP dual fluorescent system

Agarose gel electrophoresis

We calculated the new part’s base number to be 3134bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

K230500

DNA sequencing[edit] The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further

K230500
K230500


Characterization map

K230500

Fig.The Growth curve of E.coil.

K230500

Fig.The GFP curve of E.coil.

K230500

Fig.The RFP curve of E.coil.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2846
    Illegal AgeI site found at 2958
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1844