Difference between revisions of "Part:BBa K2273034:Design"

 
(→‎Source)
 
(2 intermediate revisions by 2 users not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
RFC25
+
This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:<br>
−
 
+
<table>
−
 
+
<tr>
 +
<td width="70">Prefix with</td>
 +
<td width="200"><span style="color:blue">EcoRI</span>, <span style="color:green">NotI</span>, <span style="color:red">XbaI</span>, <span style="color:orange">NgoMIV</span> and <span style="color:silver">SD</span></td>
 +
<td><span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>T<span style="color:silver">AAGGAGG</span>TCAAAA<u>ATG</u><span style="color:orange">GCCGGC</span></td>
 +
</tr>
 +
<tr>
 +
<td>Suffix with</td>
 +
<td><span style="color:orange">AgeI</span>, <span style="color:red">SpeI</span>, <span style="color:green">NotI</span> and <span style="color:blue">PstI</span></td>
 +
<td><span style="color:orange">ACCGGT</span><u>TAA</u>T<span style="color:red">ACTAGT</span>A<span style="color:green">GCGGCCG</span><span style="color:blue">CTGCAG</span>A</td>
 +
</tr>
 +
</table>
 +
Sites of restriction enzymes generating compatible overhangs are indicated by sharing one color. (<span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> are marked in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red and <span style="color:orange">AgeI</span>  and <span style="color:orange">NgoMIV</span> in orange. Additionally, the <span style="color:silver">Shine-Dalgarno sequence</span> is marked in silver and the start and stop codon is underlined.)<br><br>
  
 
===Source===
 
===Source===
  
−
<i>Aequorea victoria</i>
+
<i>Discosoma sp.</i>
  
 
===References===
 
===References===
 +
Overkamp, W. et al. Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging. Appl. Environ. Microbiol. 79, 6481–6490 (2013).

Latest revision as of 11:26, 31 October 2017


mCherry BSU


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:

Prefix with EcoRI, NotI, XbaI, NgoMIV and SD GAATTCGCGGCCGCTTCTAGATAAGGAGGTCAAAAATGGCCGGC
Suffix with AgeI, SpeI, NotI and PstI ACCGGTTAATACTAGTAGCGGCCGCTGCAGA

Sites of restriction enzymes generating compatible overhangs are indicated by sharing one color. (EcoRI and PstI are marked in blue, NotI in green, XbaI and SpeI in red and AgeI and NgoMIV in orange. Additionally, the Shine-Dalgarno sequence is marked in silver and the start and stop codon is underlined.)

Source

Discosoma sp.

References

Overkamp, W. et al. Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging. Appl. Environ. Microbiol. 79, 6481–6490 (2013).