Difference between revisions of "Part:BBa K2269027"

Latest revision as of 10:06, 15 October 2017

Tnos:YFP:Assembly Reporter PB:Luc:Tnos

Transcriptional unit composed of the 35s inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB). In normal basis, a YFP will be expressing and only when RDF appears, luciferase will be expressed.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 819
Illegal XbaI site found at 506
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 819
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 819
Illegal BglII site found at 302
Illegal BglII site found at 542
Illegal BglII site found at 649
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 819
Illegal XbaI site found at 506
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 819
Illegal XbaI site found at 506
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2269027 short</partinfo>
-Coding sequence corresponding to Flowering Locus T from Arabidopsis thaliana under the control of a chimeric promoter containing Etr8 (an E-responsive operator motif) fused to the minimal CMV promoter (Müller et al., 2014). FT has been optimized for Nicotiana benthamiana in order to promote floral induction in response to day length (Andres et al., 2015).
+Transcriptional unit composed of the 35s inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB). In normal basis, a YFP will be expressing and only when RDF appears, luciferase will be expressed.
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