Difference between revisions of "Part:BBa K2269008"
 
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<partinfo>BBa_K2269008 short</partinfo>
 
<partinfo>BBa_K2269008 short</partinfo>
  
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Firefly luciferase reporter gene regulated by a chimeric promoter containing Etr8 (an E-responsive operator motif) fused to the minimal CMV promoter (Müller et al., 2014)
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Middle part for assembly of site-specific recombination based Registers. Composed of the 35S promoter inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL).
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2269001 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2269008 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2269001 parameters</partinfo>
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<partinfo>BBa_K2269008 parameters</partinfo>
 
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Latest revision as of 16:14, 26 October 2017


Register Assembly RL YFP:Luc

Middle part for assembly of site-specific recombination based Registers. Composed of the 35S promoter inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL).



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2692
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2692
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2692
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2692
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3568