Difference between revisions of "Part:BBa K2442400"

Latest revision as of 03:28, 2 November 2017

B0032 + GFP split C-term

A schematic representation of a final split-GFP AND-gate

This is a composite of the GFP C-term split subunit (GFP2) K2442397 and the well characterised "weak" ribosome binding site B0032.

Despite the sequence error in the original K2442397 part, this composite was used for assembly of larger composite split-GFP AND-gate constructs.

K2442406 [split GFP module 2] lacI regulated promoter + RBS + GFP C-term + double terminator
K2442408 Full split-GFP AND-gate circuit [Split GFP module 1 + Split GFP module 2]

Sequence and Features

Assembly Compatibility:

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K2442400 short</partinfo>
-...
+====This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.====
+[[Image:Glasgow_gfp_gate.png|450px|thumb|center|A schematic representation of a final split-GFP AND-gate]]
+This is a composite of the GFP C-term split subunit (GFP2) [https://parts.igem.org/Part:BBa_K2442397 K2442397] and the well characterised "weak" ribosome binding site [https://parts.igem.org/Part:BBa_B0032 B0032].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Despite the sequence error in the original [https://parts.igem.org/Part:BBa_K2442397 K2442397] part, this composite was used for assembly of larger composite split-GFP AND-gate constructs.
+*[https://parts.igem.org/Part:BBa_K2442406 K2442406] [split GFP module 2] lacI regulated promoter + RBS + GFP C-term + double terminator
+*[https://parts.igem.org/Part:BBa_K2442408 K2442408] Full split-GFP AND-gate circuit [Split GFP module 1 + Split GFP module 2]
 <!-- -->