Difference between revisions of "Part:BBa K2255006"

Latest revision as of 12:17, 6 October 2017

EPS-Depolymerase (Rfc25)

Putative tail fiber protein/putative EPS Depolymerase (DIBBI_029) is an enzyme of 852 amino acids from the phage vB_XveM_DIBBI which contains two functions, one structural and one pectin lyase. Xanthomonas is a close relative of Xylella fastidiosa.

This part was designed with the Freiburg (Rfc25) extension. Thus, it contains the restriction sites NgoMIV and AgeI that are compatible and allow the absence of a start and stop codon, which ease the assembly of multiple protein domains.

Usage and Biology

This enzyme has been created to disrupt the biofilm of X. fastidiosa, see our wiki for more information about our [http://2017.igem.org/Team:Aix-Marseille/DEPS biofilm part].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2255006 short</partinfo>
-Putative tail fiber protein/putative EPS Depolymerase (DIBBI_029) is an enzyme of 852 amino acids, from the phage vB_XveM_DIBBI which contain two function one structural and one pectin lyase. ''Xanthomonas'' is a close relative of ''Xylella fastidiosa''.
+Putative tail fiber protein/putative EPS Depolymerase (DIBBI_029) is an enzyme of 852 amino acids from the phage vB_XveM_DIBBI which contains two functions, one structural and one pectin lyase. ''Xanthomonas'' is a close relative of ''Xylella fastidiosa''.
-This part was design with Freiburg (RFC[25]) extension. Thus, it contain the restriction site  NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.
+This part was designed with the Freiburg ([https://parts.igem.org/Assembly_standard_25 Rfc25]) extension. Thus, it contains the restriction sites NgoMIV and AgeI that are compatible and allow the absence of a start and stop codon, which ease the assembly of multiple protein domains.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This enzyme has been created to disrupt the biofilm of ''X. fastidiosa'', see our wiki for more information about our [http://2017.igem.org/Team:Aix-Marseille/DEPS biofilm part].
 <!-- -->