Difference between revisions of "Part:BBa K2255007"

Latest revision as of 09:27, 9 October 2017

Signal sequence of p3 from M13 (Rfc25)

The signal sequence is crucial for the excretion of p3 in the periplasm. We chose to use the one coming from M13 as we use E. coli to produce our phage. In order to be functional, the signal peptide must be cut down from the rest of the protein. Thus, we must add the cleavage site.

This part was design with the Freiburg (Rfc25) extension. Thus, it contains the restriction sites NgoMIV and AgeI that are compatible and allow the absence of a start and stop codon, which eases the assembly of multiple protein domains.

Usage and Biology

This biobrick was created to be associated with attachment protein biobricks from our collection. To see all the part from this collection, you can check out the first part of this collection: BBa_K2255008 and our [http://2017.igem.org/Team:Aix-Marseille/M13_Design design page].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2255007 short</partinfo>
-The signal sequence is crucial for the excretion of p3 in the periplasm. We choose to use the one coming from M13 as we use ''E. coli'' to produce our phage. In order to be functional, the signal peptide must be cut down from the rest of the protein. Thus, we must add the cleavage site.
+The signal sequence is crucial for the excretion of p3 in the periplasm. We chose to use the one coming from M13 as we use ''E. coli'' to produce our phage. In order to be functional, the signal peptide must be cut down from the rest of the protein. Thus, we must add the cleavage site.
+This part was design with the Freiburg ([https://parts.igem.org/Assembly_standard_25 Rfc25]) extension. Thus, it contains the restriction sites NgoMIV and AgeI that are compatible and allow the absence of a start and stop codon, which eases the assembly of multiple protein domains.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This biobrick was created to be associated with attachment protein biobricks from our collection. To see all the part from this collection, you can check out the first part of this collection: [https://parts.igem.org/Part:BBa_K2255008 BBa_K2255008] and our [http://2017.igem.org/Team:Aix-Marseille/M13_Design design page].
 <!-- -->