Difference between revisions of "Part:BBa I732096"
m |
m |
||
Line 2: | Line 2: | ||
<partinfo>BBa_I732096 short</partinfo> | <partinfo>BBa_I732096 short</partinfo> | ||
− | Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and GFP | + | Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and GFP. The activity of inserted promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay. |
* It's named as DPv2C as on pSB1A3 plasmid. | * It's named as DPv2C as on pSB1A3 plasmid. |
Latest revision as of 14:40, 14 October 2007
Promoter Activity Reporter (LacZ-alpha and GFP)
Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and GFP. The activity of inserted promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.
- It's named as DPv2C as on pSB1A3 plasmid.
- It's named as DPv2F as on pSB3K5 plasmid.
- It's named as DPv2N as on pSB3C5 plasmid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3
Illegal BamHI site found at 12 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 949