Difference between revisions of "Part:BBa I732094"
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Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and GFP-AAV. The activity of inserted promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay. | Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and GFP-AAV. The activity of inserted promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay. | ||
− | It's named as DPv2A as on pSB1A3 plasmid. | + | * It's named as DPv2A as on pSB1A3 plasmid. |
− | It's named as DPv2D as on pSB3K5 plasmid. | + | * It's named as DPv2D as on pSB3K5 plasmid. |
− | It's named as DPv2L as on pSB3C5 plasmid. | + | * It's named as DPv2L as on pSB3C5 plasmid. |
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Latest revision as of 14:38, 14 October 2007
Promoter Activity Reporter (LacZ-alpha and GFP-AAV)
Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and GFP-AAV. The activity of inserted promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.
- It's named as DPv2A as on pSB1A3 plasmid.
- It's named as DPv2D as on pSB3K5 plasmid.
- It's named as DPv2L as on pSB3C5 plasmid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3
Illegal BamHI site found at 12 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 949