Difference between revisions of "Part:BBa K2457003:Design"

 
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===Design Notes===
 
===Design Notes===
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Gibson Assembly
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This sequence is, 1130 bp of lenght, based on Escherichia coli strain MG1655 (Zhao 2016). This RecA is E. coli codon optimized, by changing codons from EcoRI and NotI sites in the codifying sequence of RecA, without removing the phase gene.
  
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===References===
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Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal G. Copeland, and Donald L. Court. An efficient recombination system for chromosome engineering in Escherichia coli. PNAS 2000 97 (11) 5978-5983; May 16, 2000
  
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GENETIC ENGINEERING USING HOMOLOGOUS RECOMBINATION1 Donald L. Court, James A. Sawitzke, and Lynn C. Thomason. Annu. Rev. Genet. 2002. 36:361–88
  
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===Source===
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Dongdong Zhao, Shenli Yuan, Bin Xiong, Hongnian Sun, Lijun Ye, Jing Li, Xueli Zhang and Changhao Bi .Development of a fast and easy method for Escherichia coli genome editing with CRISPR/ Cas9. 2016.
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Source
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===References===
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Latest revision as of 02:03, 2 November 2017


Standardized RecA coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 508
    Illegal AgeI site found at 1012
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is, 1130 bp of lenght, based on Escherichia coli strain MG1655 (Zhao 2016). This RecA is E. coli codon optimized, by changing codons from EcoRI and NotI sites in the codifying sequence of RecA, without removing the phase gene.

References

Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal G. Copeland, and Donald L. Court. An efficient recombination system for chromosome engineering in Escherichia coli. PNAS 2000 97 (11) 5978-5983; May 16, 2000

GENETIC ENGINEERING USING HOMOLOGOUS RECOMBINATION1 Donald L. Court, James A. Sawitzke, and Lynn C. Thomason. Annu. Rev. Genet. 2002. 36:361–88

Dongdong Zhao, Shenli Yuan, Bin Xiong, Hongnian Sun, Lijun Ye, Jing Li, Xueli Zhang and Changhao Bi .Development of a fast and easy method for Escherichia coli genome editing with CRISPR/ Cas9. 2016.