Difference between revisions of "Help:Materials/Antibiotic Stocks"
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Latest revision as of 19:41, 21 July 2017
- Registry Help Pages:
- TOC
- At-a-Glance
- FAQ
At iGEM HQ we prepare 1000x stock solutions of antibiotics which are stored at -20C until use. Antibiotics are also color coded.
Ampicillin
- Stock: 100mg/ml in 50% ethanol
- Working: 100ug/ml
- Color Code: Orange
Chloramphenicol
- Stock: 35mg/ml in 100% ethanol
- Working: 35ug/ml
- Color Code: Green
Kanamycin
- Stock: 35mg/ml in distilled water
- Working: 35ug/ml
- Color Code: Red
- Notes: Stocks are stored in 1ml aliquots, and thawed before use
Tetracycline
- Stock: 15mg/ml in 50% ethanol
- Working: 15ug/ml
- Color Code: Yellow
- Notes: Light-sensitive! Store in light-sensitive bottles or wrap with aluminum foil.