Difference between revisions of "Part:BBa M50037:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | This GBP sequence was optimized for use in E. coli. Also, start and stop codons were removed to facilitate fusion to the other components of our construct. | + | This GBP sequence was optimized for use in <i>E. coli</i>. Also, start and stop codons were removed to facilitate fusion to the other components of our construct. |
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===Source=== | ===Source=== | ||
− | This GBP comes from the genomic sequence of Thermus thermophilus. The complete bacterial genome was published by Henne et al. in Nature Biotechnology in 2004 (https://www.ncbi.nlm.nih.gov/pubmed/15064768), and the particular GBP was characterized in a 2006 study by Cuneo et al. in the Journal of Molecular Biology (https://www.ncbi.nlm.nih.gov/pubmed/16904687). | + | This GBP comes from the genomic sequence of <i>Thermus thermophilus</i>. The complete bacterial genome was published by Henne et al. in Nature Biotechnology in 2004 (https://www.ncbi.nlm.nih.gov/pubmed/15064768), and the particular GBP was characterized in a 2006 study by Cuneo et al. in the Journal of Molecular Biology (https://www.ncbi.nlm.nih.gov/pubmed/16904687). |
===References=== | ===References=== | ||
+ | Henne et al. in Nature Biotechnology in 2004 - https://www.ncbi.nlm.nih.gov/pubmed/15064768 <br> | ||
+ | Cuneo et al. in the Journal of Molecular Biology - https://www.ncbi.nlm.nih.gov/pubmed/16904687 |
Latest revision as of 02:51, 12 December 2016
Glucose Binding Protein of Thermus thermophilus
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This GBP sequence was optimized for use in E. coli. Also, start and stop codons were removed to facilitate fusion to the other components of our construct.
Source
This GBP comes from the genomic sequence of Thermus thermophilus. The complete bacterial genome was published by Henne et al. in Nature Biotechnology in 2004 (https://www.ncbi.nlm.nih.gov/pubmed/15064768), and the particular GBP was characterized in a 2006 study by Cuneo et al. in the Journal of Molecular Biology (https://www.ncbi.nlm.nih.gov/pubmed/16904687).
References
Henne et al. in Nature Biotechnology in 2004 - https://www.ncbi.nlm.nih.gov/pubmed/15064768
Cuneo et al. in the Journal of Molecular Biology - https://www.ncbi.nlm.nih.gov/pubmed/16904687