Difference between revisions of "Part:BBa M50028:Design"

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===Design Notes===
 
===Design Notes===
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We did not have to make any alterations of the original DNA sequence. We used Gene Designer 2.0 to analysis the original sequence and there was no indication of codon optimization, etc.
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We did not have to make any alterations of the original DNA sequence. We used Gene Designer 2.0 to analyze the original sequence and there was no indication of codon optimization, etc. Our inspiration for this project stemmed from reading the literature about possible sources for possible endogenous pathways for the control of bacterial growth. Some papers described quorum sensing pathways, specifically Autoinducer-2, as having metabolic and growth control properties (See reference 1).
  
 
===Source===
 
===Source===

Latest revision as of 07:51, 12 December 2016


LsrR Repressor Protein for the Autoinducer-2 Pathway in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We did not have to make any alterations of the original DNA sequence. We used Gene Designer 2.0 to analyze the original sequence and there was no indication of codon optimization, etc. Our inspiration for this project stemmed from reading the literature about possible sources for possible endogenous pathways for the control of bacterial growth. Some papers described quorum sensing pathways, specifically Autoinducer-2, as having metabolic and growth control properties (See reference 1).

Source

The genetic sequence was sourced from Uniprot under the name P76141 (LSRR_ECOLI).

References

1. Xavier KB and Bassler BL. 2005. Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli. J Bacteriol. 187(1): 238-248.